FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l08n02_ogla_74.3520000008cf1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l08n02_ogla_74.3520000008cf1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24566394
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG134750.020.1965561
CCTATAC141000.017.6170853
CTATACT171650.017.2936534
TATACTG138750.016.0884955
TATAGGG139100.015.5358742
GTATATA307850.015.2583861
CCCTATA129750.014.751942
GTATTGG152650.014.623541
TATACCG81900.014.6139355
GTATTAG110250.014.2163081
GTATTAT168800.014.068541
TATACTA229250.014.0258735
TAATACT177550.013.9904474
TAGACTG81050.013.5951995
ACTGTGC110050.013.0760528
TATACAG138150.013.0642145
ACAGTGC131900.012.9262848
GTATAGA126400.012.9259611
GTAATAC101500.012.6810353
TCTATAC112050.012.6739153