FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l08n02_ogla_73.3520000008cf93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l08n02_ogla_73.3520000008cf93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23872333
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG129100.017.87981
TATACTG133500.016.6856585
CCTATAC131000.015.5175883
CTATACT171250.015.4758814
GTATATA269750.014.7548651
GTATAAG104250.013.6677761
TTATACT165150.013.4304334
TATACAG138550.013.3007765
TATACCG83850.012.7447465
GTATTAT159350.012.6972651
TATAGGG122100.012.5643352
GTATTAG103950.012.4735711
TATACTA214050.012.33704955
TAGACTG77300.012.165715
ATTATAC149000.012.0491753
GTATAGA116000.011.9967141
GTATTGG147600.011.9704151
CCCTATA121200.011.9522552
GTGTAGG99900.011.933311
TAATACT163600.011.9319184