FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l08n02_ogla_71.3520000008d025.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l08n02_ogla_71.3520000008d025.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28416542
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG180250.016.5745145
TATACCG92000.014.713725
GTATATA368750.014.6967261
GTATTGG254550.014.64751
GTATAGG121800.014.3895111
GTATAAG130200.014.2272391
TTATACT215900.013.9916824
TATACAG169950.013.7781585
TAGACTG94950.013.456215
ACTGTGC129600.013.4490658
GTATAGA153550.013.3629061
TACACTG129900.013.2728115
GTATTAT214200.013.060591
ATAATAC212200.012.9822383
TACACCG95950.012.9199545
CTATACT181300.012.9155994
GTATAAT195700.012.7662071
GTATTAG139250.012.6204111
ATTATAC198350.012.4519713
TATTGGG288250.012.4077382
Produced by FastQC (version 0.11.2)