FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l08n01_ogla_77.3510000008cd98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l08n01_ogla_77.3510000008cd98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20710531
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG133550.015.6124015
GTATTGG196700.015.3382121
GTATATA279250.014.7853611
GTATAAG93700.014.1471821
TAATACT161800.014.0316914
GTATTAT150600.013.9760391
TATACCG67150.013.8630625
GTATAGA107950.013.7321111
GTATAGG86600.013.6611361
TTATACT161350.013.5703984
TACACTG94950.013.3556615
TAGACAG75450.013.2193215
CTATACT132650.013.212344
TCTATAC105000.013.1180523
TAGACTG67550.013.0778615
TATACAG114000.013.040335
ATACTGT133900.012.9109866
CCTATAC88750.012.73706153
TGCGTTC138150.012.5137669
ATAATAC149600.012.445583