FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l08n01_ogla_74.3510000008cf1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l08n01_ogla_74.3510000008cf1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24566394
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG136450.020.0220011
CTATACT172850.017.833894
CCTATAC142750.017.0691573
TATACTG143950.016.2337865
GTATATA313350.015.0264731
CCCTATA134550.014.7916052
TAGACTG80350.014.7781645
TATAGGG133600.014.292382
GTATTGG150950.014.0383121
GTATTAT166850.013.9534951
TATACTA235550.013.9335365
TATACAG143400.013.7787735
TATACCG79150.013.3219695
TTATACT176450.013.2707684
TCTATAC116200.013.161963
ACTGTGC115250.012.9400948
GTATAAG109550.012.7511131
GTATATG197500.012.6781721
TAATACT179000.012.6040874
ATAATAC165450.012.6028433