FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l07n02_ogla_85.3520000008cde5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l07n02_ogla_85.3520000008cde5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19696905
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG128750.016.19625
GTATTGG182400.014.4799021
GTATATA258850.014.4057941
TATACCG66700.014.3853825
GTATAGG88350.014.0867381
TTATACT153300.013.4165734
GTATTAT146900.013.22565651
ATTATAC140700.013.1329533
GTATAAG91550.013.1273751
TAGACTG63100.012.8724925
TAATACT151550.012.7252394
ATACTGT131800.012.6859536
CCTATAC89350.012.6528773
TACACTG86400.012.6447415
GTATAGA109350.012.6412481
TATACAG114800.012.5784635
GTGTAGG83650.012.5499511
CTATACT128800.012.3913684
GTATTAG105500.012.2920941
TACACAG86900.012.2440215
Produced by FastQC (version 0.11.2)