FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l07n02_ogla_84.3520000008ce6d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l07n02_ogla_84.3520000008ce6d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14027871
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG90300.015.6224535
GTATAGG62750.014.8366311
GTATTGG129000.014.39726451
TATACCG46750.013.8177665
TAGACTG44900.013.5407955
GTATAAG65350.013.5194891
TTATACT113250.013.5051134
GTATTAT109400.013.3729231
CCTATAC65050.013.1435753
GTATATA197750.013.0429631
TATACAG85550.012.4367975
GTATTAG76800.012.4316221
GTGTAGG60600.012.3060871
CTATACT93700.012.1154314
GTATAGA79700.011.9792781
GTATAAT101700.011.9567171
GTCTAGG45350.011.9404441
ATACTGT91000.011.8485446
ATAATAC107700.011.6874153
TAATACT119950.011.68164
Produced by FastQC (version 0.11.2)