FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l07n02_ogla_83.3520000008cee4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l07n02_ogla_83.3520000008cee4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15638679
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG98850.015.3288135
GTATAGG70050.014.5794611
GTATATA208950.014.344921
TATACAG93750.014.1866965
CCTATAC70150.013.8813973
GTATAAG70700.013.7063531
GTATTAG76050.013.6165911
TACACTG71900.013.4770795
GTATAGA84050.013.3943551
CTATACT101950.013.3251874
GTATTAT116400.013.2221841
CCTAGAC51300.013.1485543
TATACCG53500.013.1402145
GTATTGG138800.013.0732951
CTAGTAC109100.013.0618143
TAATACT120500.012.53529554
GTACCGT40100.012.4376816
GTATTAA103900.012.3440991
GTCCTAG57750.012.33816051
TATTATA169400.012.2540372