FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l07n02_ogla_81.3520000008cf6c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l07n02_ogla_81.3520000008cf6c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21219435
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG126550.016.4026625
GTATAGG93350.015.2658661
GTATATA269300.014.9580161
TATACCG65750.014.5209625
GTATTGG195200.014.4794381
TATACAG121600.013.9844235
TACACTG97850.013.8835425
TTATACT153050.013.873034
GTATAAG93550.013.8114621
GTATTAG100000.013.6331571
CCTATAC93400.013.6299823
TAATACT158950.013.5373864
CTATACT133500.013.3783864
ATACTGT129200.013.2720736
GTATTAT149900.013.2461351
ACTGTGC97950.013.0925348
TACACCG73700.013.083495
TAGACTG69250.012.6895745
ATAATAC145300.012.6518133
ATTATAC142050.012.6403173