FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l07n02_ogla_80.3520000008d00b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l07n02_ogla_80.3520000008d00b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19663821
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG122750.016.2521345
GTATAGG89000.015.3175741
GTATATA272150.015.0451671
TTATACT151750.013.8662424
GTATTAT146500.013.68273451
TACACTG92350.013.5784335
GTATAAG91100.013.5566711
GTATTGG174400.013.536551
TATACCG60900.013.4150965
ATACTGT127850.013.2261026
ATAATAC147100.013.2070043
TATACAG115900.013.1554065
CTATACT129550.013.0525784
GTATTAG98700.012.8015511
ACTGTGC92700.012.7066598
CTACACT92150.012.6285794
GTATAAT146200.012.6061491
TAGACAG72500.012.5789915
TCTATAC99400.012.567913
ATTATAC133850.012.5270843
Produced by FastQC (version 0.11.2)