FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l07n02_ogla_78.3520000008d09f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l07n02_ogla_78.3520000008d09f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20387775
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG65350.015.6998945
GTATATA270550.015.5030711
TATACTG130950.015.3797375
GTATAGG91500.014.9511971
TATACAG117450.014.599665
GTATTGG183500.014.4703911
GTATTAT147350.014.2487471
CCTATAC88200.013.6793163
GTATAAG93900.013.6584911
TTATACT155500.013.5320314
TAGACTG70450.013.3497395
CTATACT130050.013.2947654
TAGACAG73950.012.9748325
TCTATAC101950.012.9060033
GTATTAG106200.012.7474961
TAATACT164150.012.73214154
ACTGTGC89850.012.6868568
TACACTG99900.012.6475015
GTATTAA140600.012.6354531
GTATAGA111400.012.5788631