FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l07n01_ogla_88.3510000008cd61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l07n01_ogla_88.3510000008cd61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18182813
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG111550.016.0948165
TATACCG66850.015.9861875
GTATTGG195200.015.5053321
GTATAGG80200.014.6926031
GTATTAT135300.014.0470031
CTATACT115500.013.9827244
TTATACT136650.013.4522894
GTATTAG93100.013.4223171
TAGACTG59200.013.2381115
TCTATAC91550.013.1787013
GTATATA244750.013.1621991
TATTGGG217200.013.0565862
GTATAAG82950.013.0026211
TATACAG101800.012.9706215
GTATTAA123250.012.8760011
ATAATAC132550.012.7217213
GTATAAT129100.012.6973861
TGCGTTC133450.012.6344989
ATTATAC126650.012.4892463
GGTGCGT154050.012.4865257