FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l07n01_ogla_85.3510000008cde8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l07n01_ogla_85.3510000008cde8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19696905
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG127900.017.416675
TATACCG66650.016.247915
GTATAGG89950.015.0016021
GTATTGG184550.014.72661
GTATAAG89550.014.43191051
GTATTAT150750.014.3723571
ACTGTGC89550.013.6835668
GTATATA264900.013.560021
CCTATAC86750.013.415093
GTATTAG100700.013.3057741
TTATACT149300.013.0123934
TAGACTG61700.013.0096125
ATTATAC139950.012.6599763
ATACTGT126550.012.61020856
CTATACT131950.012.5994714
ATAATAC146950.012.4448063
TAATACT158350.012.2987074
TACACAG82300.012.1771435
GTGTAAG59450.012.0682851
TACACTG92500.012.0667115
Produced by FastQC (version 0.11.2)