FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l07n01_ogla_83.3510000008cee7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l07n01_ogla_83.3510000008cee7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15638679
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG98750.016.6413175
GTATAAG73350.014.832651
TATACCG55150.014.7264925
TATACAG90300.014.67456155
GTATATA215950.014.3882491
GTATTAT113800.014.3197431
GTATAGG69450.014.2290041
ACTGTGC69050.013.9628078
GTATAGA86300.013.7079321
GTATTGG139950.013.6469791
CTATACT103250.013.4790164
ATAATAC110500.013.3684643
TACACTG70150.013.2701895
TAATACT121750.013.2254774
GTATTAA104950.013.0374741
TTATACT116200.012.8761214
GTATTAG78950.012.8177221
ATTATAC109800.012.8047993
TACACCG50350.012.5458725
GTATTAC69750.012.5330561