FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l07n01_ogla_81.3510000008cf6f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l07n01_ogla_81.3510000008cf6f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21219435
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG133950.016.1690435
GTATAGG92950.015.897611
GTATTGG191800.014.5167661
TATACCG68300.014.3950055
GTATATA261300.014.1468921
TAATACT156150.013.96248154
CTATACT135650.013.6213994
TAGACTG68050.013.5405365
ATAATAC150150.013.5081693
CCTAGAC76200.013.4645773
CCTATAC96000.013.4088643
TACACCG72200.013.2227295
GTATTAG103650.012.9729281
ACTGTGC94150.012.9646168
TTATACT156250.012.9503494
TCTATAC103050.012.9063653
GTATAAG94850.012.8241
GTATAAT145400.012.7771271
TATACAG123200.012.7608465
GTATTAT147550.012.5265441