FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l07n01_ogla_80.3510000008d00e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l07n01_ogla_80.3510000008d00e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19663821
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG125300.017.3609645
GTATAGG93700.014.9084241
TATACAG117500.014.7541585
GTATATA268500.014.528571
GTATAAG93250.014.21606351
TTATACT154350.014.0021974
TATACCG63200.013.9783315
CTATACT127550.013.7043614
GTATTGG172300.013.59520151
ATTATAC139500.013.4838383
GTATAAT138550.013.4775221
GTATTAT147900.012.8182511
TACACTG90050.012.816875
ACTGTGC91000.012.7871958
GTATTAG103950.012.7527461
TACACCG65600.012.74295
TAGACTG63750.012.7401735
CCTATAC88550.012.6058433
ATAATAC147800.012.5659373
ATACTGT130950.012.4405396
Produced by FastQC (version 0.11.2)