FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l07n01_ogla_78.3510000008d092.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l07n01_ogla_78.3510000008d092.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20387775
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG128650.016.7243615
GTATAAG95300.015.6056161
GTATATA280650.015.0171691
TATACCG66750.014.9427045
TAGACTG68250.014.2663335
GTATTAT148950.014.0997451
GTATTAG102800.014.0973081
TTATACT162000.013.9276244
GTATTGG179550.013.6550651
CTATACT134050.013.3235344
TATACAG117100.013.1821925
ATACTGT135550.013.0344546
TACACTG93500.013.0043755
GTATAAT151350.012.80876351
TCTATAC100550.012.6133923
TAATACT162800.012.5462084
GTATAGG86500.012.5241491
GTATAGA112000.012.3453791
TACACAG90050.012.2367315
ATAATAC149700.012.1846243