FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l06n02_ogla_97.3520000008ccf3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l06n02_ogla_97.3520000008ccf3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22126982
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG140350.017.5978225
TATACCG75400.015.1184695
TATACAG123050.014.7837675
GTATAGG102200.014.7794381
GTATATA295150.014.7412531
GTATAAG94800.014.6805041
CTATACT138300.014.6647824
CCTATAC100200.014.315783
GTATTGG201800.014.1225061
GTATTAT160150.013.4651311
ACTGTGC103450.013.3601338
CCTAGAC73350.013.2748473
TAGACTG66900.013.0635035
GTATAAT156800.012.9954981
GTATTAG114000.012.9163111
TTATACT166050.012.7289044
TAGACAG87050.012.7132065
TCTATAC110550.012.6317753
TACACCG73900.012.5973615
GTATAGA118950.012.2989471
Produced by FastQC (version 0.11.2)