FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l06n02_ogla_96.3520000008cd2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l06n02_ogla_96.3520000008cd2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26240646
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG156900.015.1970435
GTATAAG117750.014.9660161
GTATAGG108000.014.8217451
GTATATA340500.014.6615131
GTATTGG221050.014.289741
CCTATAC114800.014.23327353
TAGACTG84800.014.2270945
CTATACT160950.013.545684
TATACCG81800.013.4714095
GTATTAT194100.013.3371851
TATACAG145400.013.3282785
ACTGTGC116250.013.1965038
TTATACT197400.012.776934
GTATAGA140350.012.7591611
TACACTG113100.012.7250165
TCTATAC131200.012.70754053
ACTGCGC55900.012.6597118
GTATTAA176850.012.6505391
CCTAGAC82050.012.3307433
GTGTAGG103650.012.2817061