FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l06n02_ogla_95.3520000008cd45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l06n02_ogla_95.3520000008cd45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20531097
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG137150.017.6620245
TATACCG64150.016.8812375
GTATATA290150.015.1919191
GTATAGG91250.014.93950751
GTATTGG206800.014.6999771
CTATACT143850.014.52817254
GTATAAG98050.014.194081
TAGACAG72150.013.6928255
TATACAG122250.013.6371715
TAGACTG70050.013.5608825
GTATTAT161150.013.4112211
TCTATAC109950.013.305693
TACACTG94400.013.1321125
TTATACT164500.013.1086554
GTATAGA115000.013.0932861
ATAATAC158300.012.9623793
GTATTAG111300.012.9310771
TATTGGG224400.012.8696852
CCTAGAC62650.012.8129033
GTATAAT160300.012.7119161
Produced by FastQC (version 0.11.2)