FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l06n02_ogla_94.3520000008cdbe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l06n02_ogla_94.3520000008cdbe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20692204
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG128950.016.0595345
GTATAGG92250.015.3437811
GTATATA288550.014.8150791
TATACAG120000.014.6449655
TATACCG68950.013.7772825
GTATTAT163850.013.7408761
GTATTAG105300.013.6677421
TAGACTG68550.013.5805215
GTATTGG185800.013.5491691
CTATACT130700.013.4460274
GTATAAT153600.013.4208821
GTATAAG92950.013.2863741
TTATACT163600.013.1226934
TAATACT170150.013.0083384
CCTATAC86500.012.9589873
TACACTG97200.012.7538735
ATTATAC145900.012.6965133
TCTATAC107700.012.6132063
ATAATAC152450.012.43141653
TACACAG83350.012.0238815