FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l06n02_ogla_93.3520000008ce37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l06n02_ogla_93.3520000008ce37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18864497
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG114400.017.5208635
GTATAGG81800.016.432881
TATACCG59350.015.4456295
GTATAAG77450.014.9640431
GTATATA215750.014.530271
TATACAG100500.014.509135
GTATTGG154750.014.2112041
CCTATAC81200.014.1556783
CTATACT114600.013.7601284
GTATAAT121800.013.7270091
TAATACT131100.013.6948774
TTATACT125600.013.6895114
GTATTAT128150.013.5657251
GTATAGA92200.013.4459091
TAGACTG60650.013.3916585
TACACCG66700.013.0314955
CCTAGAC65500.012.9077343
GTATTAG89800.012.8531761
TATTATA170200.012.8373832
ATAATAC123600.012.8351023