FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l06n02_ogla_92.3520000008cebd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l06n02_ogla_92.3520000008cebd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18471973
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG112200.015.7054395
TATACCG62700.014.771895
GTATTGG170200.014.2051561
GTCTAGG75250.013.8868571
GTATAGG83650.013.8551651
GTATATA239700.013.7524231
GTATAGA99600.013.6393761
GTATAAG84550.013.4829661
ATTATAC125850.013.4739753
GTATTAG90750.013.399271
CTATACT118400.013.3988064
TTATACT136400.013.1976374
CCTATAC86750.012.9765623
ATACTGT113350.012.9059846
TATACAG108350.012.8003825
TAGACTG61350.012.6969175
GTATTAT134150.012.6405321
TACACTG85700.012.6363915
TCTATAC91150.012.4543763
GTATTAA118550.012.340621