FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l06n02_ogla_90.3520000008cf36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l06n02_ogla_90.3520000008cf36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25390306
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG156600.015.1350045
GTATATA341950.013.7796251
GTATAGG111400.013.2606111
GTATTGG226200.012.8932991
TTATACT194100.012.8472224
CTATACT162750.012.6953234
GTATTAT187250.012.6834241
GTATAAT186400.012.6648131
GTATAAG118150.012.503021
TAATACT201400.012.4994774
TATACAG147850.012.4326425
ATACTGT157950.012.4193326
GTATTAG125050.012.3828951
TCTATAC126450.012.1329623
TATACCG86400.012.039385
CCTATAC111700.011.8640843
GTATATG228050.011.6847941
GTATAGA138500.011.6605071
ATAATAC185350.011.5832123
ACTGTGC115200.011.4202678
Produced by FastQC (version 0.11.2)