FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l06n01_ogla_96.3510000008cd2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l06n01_ogla_96.3510000008cd2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26240646
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG158400.016.072655
GTATAGG117900.014.7512531
TACACTG113400.014.5761925
TATACCG81750.014.3511635
GTATTGG223200.014.285321
GTATAAG113950.014.261771
TATACAG145000.014.1512135
GTATATA349250.014.1228591
TTATACT190800.013.5926124
GTATTAT197200.013.3976771
CTATACT162150.012.8012714
GTATAGA136350.012.790061
TACACAG105400.012.708275
TAGACAG91100.012.5132685
CCTATAC110750.012.4377813
TCTATAC127900.012.3669423
ATTATAC180750.012.3248943
GTATTAG132850.012.3043471
ATAATAC192400.012.1711193
GTCTAGG91650.012.1323531