FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l06n01_ogla_95.3510000008cd48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l06n01_ogla_95.3510000008cd48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20531097
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG137350.016.392285
GTATTGG201550.015.206511
GTATAGG94600.015.0187111
GTATATA292800.014.6219851
CTATACT139250.014.3611174
TATACCG69000.013.9745115
CCTATAC89650.013.5641343
GTATTAT160500.013.500271
ACTGTGC92350.013.474218
GTATAAG96950.013.4293611
GTATTAG108600.013.2576161
TATACAG119150.013.1954335
GGTGCGT143250.013.0297737
TCTATAC110850.013.02687553
TATTGGG227950.012.9199812
GTGCGTT138450.012.8297318
TAATACT170900.012.7854814
GTATAGA114050.012.5824231
GTATAAT156700.012.5540531
TGCGTTC130700.012.50033959