FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l06n01_ogla_94.3510000008cdb1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l06n01_ogla_94.3510000008cdb1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20692204
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG135000.015.7977145
GTATAGG88350.015.7045671
GTATATA283900.014.72878551
GTATAAG95400.014.245161
TTATACT167550.014.0047644
TATACCG68700.013.9661385
TATACAG121950.013.827015
TACACTG94100.013.7801755
GTATAGA112600.013.6305631
GTATTAT161000.013.517281
GTATAAT152150.013.4603081
ATAATAC154600.013.395853
GTATTGG190900.013.39141
CTACACT94400.013.2838874
CCTATAC95350.013.0020863
ACTGTGC91750.012.9412678
ATTATAC146900.012.9016233
GTATTAG109600.012.61630251
CTATACT139500.012.5985564
TAGACTG67600.012.5773175