FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l06n01_ogla_93.3510000008ce3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l06n01_ogla_93.3510000008ce3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18864497
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG110000.017.4016635
TATACCG59400.015.912745
GTATAGG77250.015.0085971
GTATATA219700.014.9017731
GTATAAG82900.014.3869241
TATACAG106350.014.3812545
GTATTAT125000.014.2550921
GTATTAG87650.013.8783171
GTATTGG151400.013.778041
CTATACT110300.013.694354
TAGACAG68900.013.16717155
TATATAC217550.013.1221583
TAATACT132600.013.1107474
ATAATAC125150.013.0941733
CCTATAC79450.013.0930283
TTATACT126200.013.0605064
TAGACTG59150.013.00884155
GTCTAGG75200.012.953431
TATTATA167050.012.9377352
TACACTG87000.012.8300095