FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l06n01_ogla_92.3510000008ceb0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l06n01_ogla_92.3510000008ceb0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18471973
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG112700.016.0151825
GTATTGG169950.014.7624291
GTATAAG83100.014.4665371
GTATATA241100.014.1899351
GTATAGG88050.014.1389451
TAGACTG58950.014.0196665
TATACCG64700.013.9483095
GTATTAT139100.013.8689641
ATACTGT115300.013.5108136
CCTATAC85900.013.4368743
GTGTAGG84150.013.100231
TAGACAG72900.013.0960635
GTATTAC76600.013.0887691
CCTAGAC65100.012.9874593
TACACTG83900.012.7377515
CTATACT119850.012.7218714
TATACAG103100.012.6691155
GTATAAT133800.012.571651
ATTATAC131950.012.4912313
GTATTAA119950.012.3594581