FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l06n01_ogla_90.3510000008cf39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l06n01_ogla_90.3510000008cf39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25390306
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG156750.015.4844565
GTATAAG118450.014.5224861
TATACCG82550.014.269785
GTATATA346250.014.0395541
GTATAGG111650.013.449181
TAGACTG83950.013.1265295
GTATAGA145250.013.1188471
TTATACT198950.013.0598714
GTATTGG226000.013.0151631
GTATTAT194550.012.7254721
GTATAAT188600.012.547441
ATTATAC182200.012.51383
TAATACT212250.012.2638934
ATAATAC190800.012.1489233
ATACTGT164100.012.1268236
GTATTAG131050.012.0746531
TATACAG150900.011.8983085
CCTATAC110200.011.7673163
CTATACT165500.011.6239174
TACACCG85100.011.6095785
Produced by FastQC (version 0.11.2)