FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l05n02_ogla_99.3520000008cff0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l05n02_ogla_99.3520000008cff0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17685336
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG107450.016.9296155
TATACCG52450.015.7564545
GTATTGG149950.015.52045251
CCTATAC78350.015.2156143
GTATAGG75800.014.9755731
CTATACT114950.014.7094284
GTATTAG86750.014.5635391
CCTAGAC62950.014.4109423
GTATAAG76200.014.0243361
TAGACTG54700.013.9795535
GTATATA226400.013.7200631
TCTATAC89750.013.6004543
TACACAG71900.013.54190355
GTGTAAG55700.013.3874911
TAGACAG65250.013.3206475
TACACCG61450.013.216885
GTGTAGG73850.013.1843321
GTCTAGG71800.013.1638641
TTATACT126450.013.033634
TATACAG97650.012.8892725
Produced by FastQC (version 0.11.2)