FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l05n02_ogla_98.3520000008d068.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l05n02_ogla_98.3520000008d068.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24365529
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG147600.016.9901315
GTATAAG112350.016.14921
GTATAGG102250.015.7469751
CTATACT147550.015.515254
GTATTGG218750.015.437691
TATACCG75050.015.188325
GTATATA312900.014.8758321
CCTATAC104850.014.5862533
TATACAG132250.013.9343025
TACACCG80100.013.8749855
TAGACGG41100.013.8671945
TACACTG106000.013.8452825
GTATAGA129400.013.7644151
TCTATAC119200.013.7467213
CCTAGAC77600.013.7101623
TAGACTG80250.013.6123145
TGGTCGC225650.013.5377594-95
TAGACAG87100.013.4687715
TATTGGG245850.013.4462072
ACTGTGC104350.013.3806268
Produced by FastQC (version 0.11.2)