FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l05n02_ogla_102.3520000008ce87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l05n02_ogla_102.3520000008ce87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12610236
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG105700.016.984991
TATACTG80900.016.4969185
GTGTAGG57950.016.227821
GTATAGG56650.015.5941411
TATACAG69600.015.2174055
GTATAAG54200.015.15986251
TACACTG60000.014.8816685
TACACCG41150.014.6581415
TATACCG38250.014.6519565
GTATTAG64900.014.4900181
ACTGTGC55750.014.3965788
GTATATA161200.014.3781571
GTATAGA66050.013.8062851
CTACACT58400.013.744184
CTATACT77500.013.6662124
GGACCGT25750.013.6489576
TAGACTG40750.013.6365115
TGCGTTC76100.013.6036299
CCTACAC53800.013.5951853
GACTGTG35400.013.5507437