FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l05n02_ogla_101.3520000008cf0f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l05n02_ogla_101.3520000008cf0f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28233776
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG185650.016.3727045
GTATAGG124050.016.0421261
GTATTGG226300.015.65665151
TACACTG129450.015.3359125
TGGTCGC336200.015.31182894-95
TATACCG84100.015.1347145
CTATACT180300.014.9883654
CCTATAC125100.014.8443433
TAGACAG101650.014.8110985
TACACCG91700.014.7608355
CTACACT124650.014.4024784
GTATATA393200.014.1807511
GGTCGCC329000.014.10894494-95
ACTGTGC118400.014.0384848
GTCTAGG98050.013.9989311
GTGTAAG89250.013.889191
TAGACGG46100.013.805095
GTATAGA157100.013.7858411
TATACAG160550.013.7851345
GTATAAG133650.013.7526571