FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l05n02_ogla_100.3520000008cf86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l05n02_ogla_100.3520000008cf86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20289905
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG129100.016.1137455
TATACCG57600.015.7492365
GTATAAG91550.015.4082071
CTATACT125700.015.227184
TATACAG112400.015.1696925
GTATATA278550.014.6809431
GTATAGA110400.014.4982241
TACACCG64700.014.1677745
GTATAGG90800.013.9139281
GTATTGG143500.013.7356811
TGGTCGC213450.013.73327494-95
CCTACAC86500.013.6720283
CCTATAC87100.013.5778473
GTATTAG96150.013.5349051
CTACACT93650.013.2874974
TCTATAC102100.013.2112013
TACACAG86200.013.168595
TACACTG94800.013.1262775
GTATAAT143900.013.1033911
TAGACTG62550.012.9083365
Produced by FastQC (version 0.11.2)