FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l05n01_ogla_99.3510000008cff3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l05n01_ogla_99.3510000008cff3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17685336
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG57750.017.0243435
TATACTG110350.016.0111545
TACACTG81750.015.5703745
CCTATAC79750.015.3662563
GTATAGG80450.015.3544111
GTATTGG154300.015.0258751
GTATAAG81200.014.8615321
TATACAG96050.014.8346035
CTATACT109100.014.5846724
GTATATA232450.013.8575361
GTATAGA93000.013.7932541
TAGACTG58800.013.6509035
TACACAG78350.013.5787895
CCTAGAC62300.013.5709723
CTACACT81150.013.4621954
GTATTAG87250.013.1775851
TTATACT124600.013.1515714
CTTACAC54200.013.1453163
TCTATAC89700.013.0792713
TGCGTTC108900.013.0402759