FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l05n01_ogla_98.3510000008d06b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l05n01_ogla_98.3510000008d06b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24365529
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG151750.017.401815
GTATTGG218400.016.6205021
CCTAGAC77000.016.0382423
TACACCG81500.015.7928325
TATACCG75600.015.2034425
TAGACTG83250.015.0614935
TCTATAC124700.014.9311683
TATACAG132450.014.8455645
CCTATAC103100.014.7883793
GTATAGG103350.014.7569961
GTATATA313900.014.6062731
CTATACT149550.014.228634
GTATAAG109700.014.2059621
TATTGGG250150.014.108282
ACTGTGC101700.014.0099948
GTCTAGG86600.013.7708031
TTATACT178650.013.6391044
GTGTAGG97350.013.6166951
TAGACAG88750.013.5929495
ACAGTGC118200.013.581168