FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l05n01_ogla_103.3510000008ce03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l05n01_ogla_103.3510000008ce03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16297591
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG53950.017.2547175
TATACTG110150.016.9022625
GTATAGG77950.016.82221
GTGTAGG70150.016.4576721
CCTATAC75500.016.23083
CTATACT104150.015.5966534
TAGACAG62650.015.086045
TATACAG93850.014.8784025
ACTGTGC71600.014.7256748
GTATTGG134500.014.5887331
GTATAAG75750.014.4883571
TAGACGG28300.014.4329545
GTCTAGG60050.014.0830531
GTATATA223400.014.0362331
TACACTG76950.013.9489995
GTATAGA90550.013.7993021
GTATTAG79900.013.6763711
TACACCG52950.013.6339245
GTATTAT116700.013.5162751
GTCTTAG48800.013.4353521