FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l05n01_ogla_102.3510000008ce8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l05n01_ogla_102.3510000008ce8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12610236
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG75550.018.4826055
TATACCG37450.017.5015935
GTATAGG57050.016.1564621
GTATTGG103900.015.5933341
TACACTG56700.015.329165
TATACAG69400.015.1245735
TAGACAG44200.014.8288395
TACACCG41650.014.7104185
TAGACTG40400.014.6953175
GTATAAG53350.014.33811
CTATACT80350.014.3053024
CTACACT56900.014.0237634
GTATAGA66100.013.944421
TCTATAC61000.013.9377433
CCTATAC56700.013.7382073
TACACAG52600.013.7248685
GTCTAGG45700.013.6193151
GTATTAG62250.013.5856631
GTACTAG89350.013.5595661
GTGTATA70400.013.4301411