FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l05n01_ogla_101.3510000008cf02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l05n01_ogla_101.3510000008cf02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28233776
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG191450.016.9934675
TATACCG88450.015.8942735
GTATAGG123750.015.8179561
CTATACT182450.015.7498914
GTATAAG129150.015.3773051
TATACAG162050.015.2405655
CCTATAC123050.015.2084073
GTCTAGG102050.015.1310381
TAGACTG92800.015.0980515
TACACTG129600.014.9520925
GTATTGG216800.014.9459471
GTATATA401300.014.7281971
ACTGTGC119600.014.6134998
GTGTAGG122000.014.4871011
TACACAG119750.014.1592255
TCTATAC148350.014.1515443
CTACACT126650.013.9884534
TAGACAG101650.013.8302865
GTATTAG145450.013.62136651
TACACCG91100.013.6072065