FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l05n01_ogla_100.3510000008cf89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l05n01_ogla_100.3510000008cf89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20289905
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG124850.017.5377065
TATACCG62250.017.3962615
CCTAGAC68350.015.5664753
TATACAG111000.015.3614395
GTATTGG144300.015.2115361
GTATAAG88200.014.8136321
GTATAGG91600.014.6787281
CCTATAC88300.014.523923
TACACCG67350.014.456955
GTATAGA105700.014.1140651
TACACTG93000.013.9424825
CTATACT127150.013.8964584
GTATATA279200.013.8007571
ACTGTGC81750.013.7692178
ACTGCGC44550.013.7527868
TTATACT146450.013.6867594
TAGACAG72950.013.5424885
TCTATAC104300.013.4344063
TACACAG82800.013.3081625
GTCTAGG72000.013.2635871
Produced by FastQC (version 0.11.2)