FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l04n02_ogla_tva1.3520000008cfe3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l04n02_ogla_tva1.3520000008cfe3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25587062
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC263050.102805863369542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG162800.017.7114035
GTATTGG218600.016.450041
TACACTG121600.016.0556165
TATACAG148650.015.7863135
GTATAGG107300.015.4506561
CTATACT156800.015.1172924
TACACAG116500.014.5566355
TAGACTG80050.014.4199475
GTATAGA140650.014.21882251
GTATATA349200.014.2155391
TATACCG77300.014.1340635
GTATTAG127950.014.1079711
TCTATAC131450.014.0213783
CCTATAC105350.013.9780543
CTACACT124150.013.8892714
GTGTAGG116300.013.8465391
ACAGTGC126400.013.8290758
TAGACAG88650.013.8248255
TATTGGG236700.013.7672472
GTATAAG111750.013.6451641
Produced by FastQC (version 0.11.2)