FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l04n02_ogla_114.3520000008cd38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l04n02_ogla_114.3520000008cd38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26261131
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG159950.016.809325
TATACCG77450.016.0693365
GTATAGG111000.015.8771081
GTATATA350700.015.3331411
GTATAAG117650.015.302691
TAGACTG82150.015.2077925
GTATTGG232950.015.0900091
CTATACT165850.014.8079154
CCTATAC107050.014.6879153
TACACTG111750.014.6652545
GTATAGA138050.014.348951
GTGTAGG108050.013.9805031
TATACAG146250.013.8366755
GTGTATA148450.013.7596921
CCTAGAC83300.013.6292433
TCTATAC137500.013.5080573
GTCCTAG89450.013.4357111
TACACCG79950.013.3090665
GTATTAG132650.013.2857831
GTGTATG162750.013.1636421
Produced by FastQC (version 0.11.2)