FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l04n02_ogla_113.3520000008cd88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l04n02_ogla_113.3520000008cd88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29665809
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG196550.018.1500325
GTATAGG135700.017.2576921
GTATTGG274700.017.1022511
CCTAGAC103650.016.7276173
GTATAAG140100.015.8002311
TACACTG137650.015.7706835
CCTATAC137050.015.5971043
CTATACT195150.015.4810014
TAGACTG99150.014.8518145
GTGTAGG136800.014.8271411
GTATAGA164250.014.7785271
GTATATA396050.014.7286851
TCTATAC149100.014.6233043
TATACCG101150.014.5581545
CCTACAC123100.014.5090953
TAGACAG111050.014.4152385
TATTGGG304800.014.088642
TACACAG126450.014.0871435
TATACAG170300.014.0859855
TGCGTTC191400.013.7970289
Produced by FastQC (version 0.11.2)