FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l04n02_ogla_112.3520000008cdf2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l04n02_ogla_112.3520000008cdf2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23830559
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG149000.017.119835
GTATAGG102450.016.5527951
TATACCG76200.016.332695
GTATAAG106950.016.1672331
GTATTGG200500.015.7551431
TACACTG109600.015.3427825
TAGACTG75750.015.238255
GTATATA321950.015.226721
CTATACT149000.015.0475964
TACACCG76650.014.9973535
CCTATAC101600.014.7742273
GTACTAG169950.014.5623551
GTGTAGG100400.014.5251191
GTCTAGG83300.014.48450851
ACTGTGC102150.014.368238
GTATAGA127850.014.1559591
TATACAG135250.014.1188785
TCTATAC122350.014.054523
TTATACT176950.013.9056274
GTATTAG123700.013.5556411
Produced by FastQC (version 0.11.2)