FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l04n02_ogla_111.3520000008ce7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l04n02_ogla_111.3520000008ce7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30846502
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG129700.017.1037851
TATACTG187550.016.9948485
TATACCG96900.016.3242135
GTATAAG145350.016.0138721
GTATTGG254700.014.8083571
GTATATA402650.014.7585941
TACACTG136900.014.5732925
GTCTAGG100450.014.470611
CTATACT196450.014.2904954
GTATAGA167700.014.247881
TATACAG168050.014.2463575
CCTATAC135650.014.1472943
GTGTAGG121650.013.7450361
TCTATAC162350.013.6054353
GTATTAT238800.013.3078261
GTGTATG194750.013.2933481
TAGACTG99100.013.2296165
ACTGTGC133600.013.154838
TTATACT232800.013.0997754
GTCTATA132400.013.0954441
Produced by FastQC (version 0.11.2)