FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l04n02_ogla_107.3520000008cef1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l04n02_ogla_107.3520000008cef1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28594662
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG172450.016.6369065
GTATAGG130300.015.6028611
TATACCG88500.015.4578115
GTATTGG262400.014.9889561
CTATACT178850.014.9792114
TACACCG88200.014.7025575
TATACAG156750.014.3941135
GTATATA377300.014.2516291
GTATAAG135050.014.0692281
GTCTAGG97700.014.0510291
TACACTG122500.014.0369035
TCTATAC144100.013.8117553
CCTATAC122100.013.6938573
TAGACAG96550.013.5786195
TAGACTG90350.013.5640795
GTGTAGG118000.013.3244991
GTATTAT211600.013.132431
TTATACT215750.013.1218274
GTATAGA155950.013.0670271
GTATTAG140000.013.0628391
Produced by FastQC (version 0.11.2)