FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l04n02_ogla_106.3520000008cf79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l04n02_ogla_106.3520000008cf79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29550054
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG175250.016.6155555
TATACCG87850.015.8430325
GTATAGG125550.015.7773991
GTATTGG265950.015.5573011
GTATATA378450.015.1500771
TACACTG126050.015.1117215
GTATAAG130000.015.0911661
TAGACTG90850.014.6401515
TATACAG160400.014.4223825
TAGACAG103450.014.4182185
ACTGTGC122800.013.9637538
GTGTAGG120350.013.93300151
GTATTAG146050.013.7254621
CTATACT180300.013.6736364
CCTATAC125150.013.6641953
GTCTAGG93150.013.4628541
TCTATAC150500.013.4457653
TACACCG94750.013.4359385
ACAGTGC132000.013.3503698
CTACACT127100.013.3424344
Produced by FastQC (version 0.11.2)