FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l04n01_ogla_tva1.3510000008cfe6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l04n01_ogla_tva1.3510000008cfe6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25587062
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC257950.10081266852755505No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG164600.017.5438355
GTATTGG222150.016.232631
TATACCG76400.015.9146615
TATACAG152250.015.8473715
ACTGTGC112150.015.6689728
GTATAGG108150.015.6392851
TACACTG124350.015.6217015
CCTATAC102900.015.27932553
CTATACT155500.015.2121484
GTATATA355100.015.0921751
TAGACAG86150.015.0507515
TACACCG85450.014.7849675
TAGACTG85750.014.6778545
GTATAGA137050.014.3520771
GTCTAGG91850.013.8110131
GTATAAG111900.013.5442211
TCTATAC127500.013.44895653
TATTGGG238900.013.4214272
TGCGTTC163750.013.3418079
GTATTAG125500.013.3257721
Produced by FastQC (version 0.11.2)