FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l04n01_ogla_114.3510000008cd3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l04n01_ogla_114.3510000008cd3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26261131
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG159950.017.370475
GTATAGG105400.015.9569781
GTATTGG227600.015.9481061
TATACAG140050.015.6674685
TACACCG82350.015.3988255
TATACCG78400.015.326555
TACACTG119750.015.3091735
TAGACTG80050.014.65465455
GTATAAG119000.014.6523441
GTATATA353150.014.5564691
GTGTAGG106450.014.4606341
GTATAGA145150.014.3365641
CTATACT158650.014.1312554
CCTAGAC82600.013.7434793
CCTATAC110400.013.6385763
TACACAG107300.013.4116695
GTATTAG134150.013.387181
TAGACAG94150.013.3679715
GTCTAGG88400.013.3286891
ACTGTGC111050.013.1722428
Produced by FastQC (version 0.11.2)